月季未成熟胚再生植株的研究

Plant regeneration from immature embryos of roses

为了建立月季遗传转化的再生体系,该试验以‘漂多斯’、‘蓝丝带’、‘索奴拉’3个月季品种的未成熟胚为外植体,获得了月季再生植株.结果表明,在MS+ZT 0.5 mg/L、MS+ZT 4.0 mg/L+NAA 1.0 mg/L、MS+ZT 0.5 mg/L+NAA0.5 mg/L培养基上,‘漂多斯’、‘蓝丝带’、‘索奴拉’能够诱导出再生芽,再生率分别为33.3%、33.3%、16.7%;再生芽在不含植物生长调节剂的MS基本培养基上伸长、展叶,在MS+IBA 0.5 mg/L的培养基上能得到生根的再生植株.随着月季未成熟胚贮藏时间的延长,不仅污染率增加,而且胚成熟后多发育成胚芽;贮藏时间不宜超过90 d.这种以未成熟胚为外植体获得再生植株的途径,可为遗传转化提供有效的受体系统.

In order to obtain rose regeneration system for genetic transformation, the immature embryos of Rosa ‘Peaudouce＇, ‘Blue Ribbon＇ and ‘Sonora＇ were taken as explants, and regenerated plants were obtained. Adventitious buds were induced in MS ＋ ZT 0.5 mg/L for ‘Peaudouce＇, MS ＋ ZT 4.0 mg/L ＋ NAA 1.0 mg/L for ‘Blue Ribbon＇ and MS ＋ ZT 0.5 mg/L ＋ NAA 0.5 mg/L for ‘Sonora＇, and the regeneration rates were 33.3%, 33.3% and 16.7% respectively. The regenerated buds grew into shoots in the MS medium without plant growth regulators, and they rooted in MS ＋ IBA 0.5 mg/L. The contamination rate increased and the matured embryos prefer to develop into plumules when the storing time prolonged ; the longest time for storing was less than 90 days. The results indicate that the plantlet regeneration system from immature embryos can be a proper receptor system for genetic transformation in roses.