芫菁科昆虫体内斑蝥素的气相色谱法测定

Gas-chromatographical study of cantharidin in Meloidae

分别采用酸水解法和直接浸提法处理不同种芫菁样品、短翅豆芫菁Epicauta aptera Kaszab的卵和大斑芫菁Mylabris phalerata Pallas的不同虫体部位,后用气相色谱仪测定斑蝥素含量。结果表明：用酸水解法处理后的芫菁体内斑蝥素含量较之用直接浸提法处理后有显著提高,增高幅度在1-9倍之间,其中以豆芫菁属Epicauta昆虫的增高幅度最大,一般在7倍以上,而斑芫菁属Mylabris的斑蝥素含量增幅不高,芫菁卵中斑蝥素含量变化不显著;斑蝥素主要富集于大斑芫菁的腹部。

The samples from different species of Meloidae, the eggs of Epicauta aptera Kaszab and the different parts of Mylabris phalerata Pallas were treated with acidolysis or direct extraction, and cantharidin contents were determined by gas chromatography. The result showed that cantharidn contents in the samples of Meloidae treated with acidolysis method were 1 to 9 times higher than those dealt with extraction method, usually more than 7 times higher in Epicauta, and no much differences in Mylabris. The differences of cantharidin contents in eggs between samples with two methods were not notable. The cantharidin was mainly found in the abdomen of M. phalerata.