小麦单倍体育种中培养基的研究

Studies on Culture Medium of Wheat Haploid Breeding

小麦单倍体育种中培养基直接影响幼苗产生率,为了找到一种适宜小麦幼胚生长的培养基,对16份F1单交组合幼胚在8种培养基上的生长发育状况进行了研究,8种培养基的幼苗平均产生率分别为32.71%、41.48%、47.15%、53.59%、57.98%、62.05%、53.26%和42.21%。培养基①、②、③、⑧的幼苗平均产生率较低,培养基④、⑤、⑥、⑦的较高,其中培养基⑥(1/2MS+0.2mg/LIAA+0.2mg/L6-BAP+2%蔗糖+7.5g/L琼脂PH=5.8)的幼苗产生率为62.05%,且幼芽分化明显,根系弯曲至瓶底,叶色深绿,较其他7种培养基培养效果最好。

Culture medium affects seedling production of wheat haploid. Growth and development of 16 single F1 on eight kinds of medium were studied in order to find a kind of the optimal culture medium for growth of wheat young embryo. Average seedling production frequency of 8 eight kinds of medium were 32.71%, 41.48%, 47.15%, 53.59%, 57.98%, 62.05%, 53.26% and 42.21%, respectively. Average seedling production frequency of culture medium （1）, （2）, （3） and （8） were lower and that of （4）, （5）, （6） and （7） were higher. Seedling production frequency of the medium （6）, （1/2MS＋0.2 mg/L IAA＋0.2 mg/L6-BAP＋2% sucrose ＋7.Sg/L agarose, PH=5.8）, was 62.05%. The young germnation differentiation of the medium （6） was obvious, and roots curved to the bottle bottom and leaf was deeper green. The results showed that the culture effect of the medium （6） was better than that of the other.