中风前抗轴突生长抑制因子DNA免疫促进脑缺血功能康复

Effect of Prestroke DNA immunization against Neurite Growth Inhibitors on the Neurological Improvement Following Permanent Local Cerebral Ischemia

目的证实中风前抗轴突生长抑制因子DNA免疫对脑缺血神经功能康复的促进作用。方法经腓肠肌注射抗轴突生长抑制因子DNA疫苗免疫动物,每周一次、共6周;血清中检测出相关抗体后,采用永久性阻断大脑中动脉的方法制备左侧局部脑缺血模型;分别采用改良的神经病严重程度记分、被动逃避试验和悬臂迷宫试验评价运动功能、认知行为和焦虑样情感的。结果中风前接受抗轴突生长抑制因子DNA免疫,局部脑缺血后出现明显的运动恢复,但是认知行为和焦虑样情感障碍则没有明显改善。结论中风前抗轴突生长抑制因子DNA免疫可促进脑缺血后的运动功能恢复。

Objective To conform the effect of prestroke NDA immunization against neurite growth inhibitors on the neurological improvement following permanent local cerebral isehemia. Methods DNA immunization was carried out by intramuscular injection of pcDNA3. 1 （ ＋ ） -neurite growth inhibitors （ pcDNA-NGIs ） into the gastrocnemius once a week for 6 weeks. Left local cerebral isehemia was permanently induced through the occlusion of the middle artery （MCAO） after pcDNA-NGIs-induced auto antibodies in sera were detected. The following program of the modified neurological severity score（ mNSS）, the passive avoidance task and the elevated plus maze task were respectively used to test the sensorimotor deficit,the cognitive behavior and the anxiety-like affective disorder. Results Prestroke DNA immunization with pcDNA-NGIs led to increased sensorimotor recovery but no marked recovery of cognitive deficit and anxietic-like change achieved following local cerebral ischmia. Conclusions Prestroke DNA immunization with pcDNA-NGIs can enhance the neurological improvement following permanent local cerebral ischemia.