泊洛沙姆在全自动血液分析仪鞘液中的应用

Use of Poloxamex of Self-made Sheath Reagent for Fully Automatic Hemocyte Analyzer

目的:研究泊洛沙姆(Poloxamex)在SE-9000全自动血液分析仪用自制鞘液中的应用。方法:用优选出的泊洛沙姆最佳浓度及其它成分自制成试验鞘液,对进口配套的全血质控物进行准确度、精密度分析,以此评价自制鞘液的性能。结果:含有泊洛沙姆成分的自制试验鞘液,对进口配套全血质控物测得的各项质量控制指标,其均值WBC:7.09G/L,RBC:4.39T/L,Hb:131g/L,HCT:38.5L/L,PLT:227G/L,MCV:87.7fl,MCH:29.9pg,MCHC:341g/L,均处于质控物允许范围内;其标准差和变异系数分别是WBC:0.16,2.3;RBC:0.05,1.1;Hb:1.15,0.88;HCT:0.34,0.88;PLT:6.6,2.9;MCV:0.44,0.5;MVH:0.26,0.87;MCHC:2.6,0.76,均低于仪器生产厂家和CLIA'88的要求;自制试验鞘液的空白背景计数值WBC:0.05G/L,RBC:0,Hb:0,PLT:3G/L,IMI:0,DIFF:0,EO:0,BASO:0,均低于仪器生产厂家的限定值。结论:含有泊洛沙姆成分的自制试验鞘液,其性能符合仪器生产厂家和CLIA'88的质量控制要求,能替代国外同类产品用于SE-9000全自动血液分析仪的配套使用。

Objective. To investigate use of poloxamex of self-made sheath reagent for fully automatic hemocyte analyzer. Methods. A series of comparisons between the self-made sheath reagent and imported reagents were carried out. Results. The value of WBC, RBC, Hb, HCT, PLT, MCV, MCH, MCHC was 7. 09G/L, 4. 39T/L, 131 g/L, 38. 5L/L, 227G/L, 87. 7 fl, 29. 9 pg, 341 g/L, respectively, was within the working range of the criteria of quality control. The standard deviation and variance coefficient were lower than the requirements of the manufacturer and Clinical Laboratory Improvement Amendment 88, respectively. The countable value met the requirements of the manufacturer. Conclusions. The self-made sheath reagents can be used to replace the imported reagent for SE-9000 fully automatic hemocyte analyzer.