65份枇杷种质资源的RAPD分析

RAPD Analysis of 65 Accessions of Loquat Germplasm (Eriobotrya spp.)

利用RAPD技术对65份有代表性的枇杷种质资源进行了遗传亲缘关系及分类研究。从所筛选的Sangon引物中选取16个10个碱基的随机引物对65份枇杷种质资源的基因组DNA进行扩增,共产生210条谱带,其中多态性谱带为210条(100%),表明65份种质资源具有丰富的多态性。各种质间的遗传距离为0.046～1.000,并根据遗传距离,利用UPGMA构建了亲缘关系树状图。在当D=0.847时,65份种质资源可分为两大组第1组为非栽培品种,包括7号(栎叶枇杷)、1号(贵州野生)、5号(台湾枇杷)和8号(海南野生),第2组为栽培品种,这与传统分类法是一致的;但在栽培组中,各分组的聚类结果,与传统的中国枇杷品种常用的分类方法有所不同。

The genetic relationship and classification of 65 accessions of loquat germplasm （Eriobotrya spp.） was analyzed by RAPD. Screened were 1 610 bp-primers from Sangon arbitrary primers to amplify the genomic DNA of 65 accessions of loquat. A total of 210 bands were amplified, among which 210 bands （100 % ） were polymorphic. The results showed polymorphism of loquat germplasm was high. The genetic distance among 65 accessions ranged from 0.046 to 1.000. According to UPGMA cluster analysis of genetic distance, a DNA molecular dendrogram was developed for 65 accessions of loquat, which were divided into 2 groups as D=0.847. Accessions No.7 （E. prinoides, E. deflexa）, No. 1 （Guizhouyesheng）, No.5 （Taiwanpipa） and No.8 （Hainanyesheng） belonged to the wild group and the other accessions to the cultivar group. This result is consistent with that of the traditional classification in the previous studies, but the classification of sub-groups of the cultivar group based on cluster analysis was somewhat different from that of the traditional classification.