增殖细胞核抗原在鼻咽癌中表达的定量研究

Quantitative observation of expression of proliferation cell nuclear antigen in nasopharyngeal carcinoma

为了增加鼻咽癌的诊断和预后判定指标，应用抗增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｅｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）单克隆抗体，采用免疫组化染色ＬＡＳ－ＳＰ法及图象分析技术，对８２例鼻咽鳞状细胞癌和２５例鼻咽粘膜炎症组织标本进行了定量研究，并对鼻咽癌患者５年生存率与ＰＣＮＡ表达情况进行了相关性分析。结果：①根据ＰＣＮＡ阳性细胞计算的鼻咽癌细胞增殖指数与鼻咽癌的病理分级呈明显的正相关，与患者５年生存率呈明显负相关。②图像处理检测的ＰＣＮＡ表达量与鼻咽癌的病理分级也呈明显的正相关，与５年生存率呈负相关，但泡状核细胞癌虽属低分化鳞癌却不符合上述规律。结论：ＰＣＮＡ能很好地反映鼻咽癌的增殖活性，在临床病理上具有应用前景。

Eighty two cases of nasopharyngeal squamous cell carcinoma and twenty five cases of nasopharyngitis were studied by means of LAS SP immunohistochemical method with monoclonal anti PCNA antibody and using image pattern analysis technique; correlation analysis was performed on expression of PCNA and five year survival rate. It was shown: ①The cell proliferative index and pathological grading of NPC were positively correlated, and the cell proliferative index and five year survival rate negatively correlated. ② PCNA expression level and pathological grading of NPC were positively correlated, and PCNA expression levels and five years survival rate negatively correlated in all pathological gradings of NPC except the vesicular nucleus cell carcinoma. The results indicate that PCNA affects proliferation activity of NPC and there are bright prospects for their application in the clinic. Expression of PCNA may also become a good indicator of the prognosis of NPC and the choice of treatment.