HIV／HCV共感染对HIV-1生物学特性影响的研究

Study on the influence of biological characteristics to HIV/HCV co-infection among HIV infected illegal blood donors in China

目的研究有偿献血人群HIV／HCV共感染对HIV-1基因离散率、病毒载量、CD4^＋T淋巴细胞等生物学特性影响。方法扩增HIV-1 env基因C2～V3区，ClustalW和Phylip软件计算基因离散率，NASBA方法检测HIV-1病毒载量，采用Coulter公司的EpicsXL流式细胞计数仪检测CD4^＋细胞数，用SPSS软件t检验分析HIV／HCV共感染对HIV-1生物学特性的影响。结果HCV共感染与非共感染人群的HIV-1平均基因离散率为7．95％、15．73％（P〈0．001），平均病毒载量为4．61、4．45（P=0．522），平均CD4^＋T淋巴细胞数为308、251（P=0．161）。结论该有偿献血人群中HIV／HCV共感染对HIV-1基因离散率有影响，而对病毒载量、CD4^＋T淋巴细胞无影响。

Objective To study the influence of biological characteristics on HCV/HIV co-infection, including HIV-1 sequence distance, HIV-1 viral load and CD4^ ＋ count. Methods HIV- 1 sequence distance was calculated by Clustal W and Phylip software while HIV-1 viral load being tested by NASBA and CD4^＋ count was tested using Epics XL of Coulter. Significance was determined by t-test using SPSS 12.0. Results The mean HIV-1 genetic distances were 7.95 % and 15.73 % （P〈 0. 001） between those with HCV co-infection and those without. Their mean HIV-1 viral loads were 4.61 and 4.45 （P = 0. 522） and their mean CD4^＋ T counts were 308 and 251（P = 0. 161）, respectively. Conclusion Data showed that in the study group, the HIV/HCV co-infection had an influence on the HIV sequence distance, but did not have major impact on HIV-1 viral load and their mean CD4^＋ T count.