西藏牦牛血清乳酸脱氢酶H亚基的遗传变异体

Genetic Variants in H Subunit of LDH in the

采用聚丙烯酰胺凝胶电泳分析西藏嘉黎和亚东地区８５头牦牛血清乳酸脱氢酶（ＬＤＨ）同工酶。结果显示，牦牛血清ＬＤＨ同工酶具有ＬＤＨ１～ＬＤＨ５五种形式，ＬＤＨ的Ｈ亚基存在Ａ、Ｂ、Ｃ三种遗传变异体，Ｃ型遗传变异体平均占８２．３５％。值得注意的是，嘉黎地区具有Ａ或Ｂ型Ｈ亚基的牦牛血清中ＬＤＨ２的相对活力显著低于具有Ｃ型Ｈ亚基的牦牛，其机制和意义有待进一步研究。

yaks from Jiali and Yadong in Tibet were used to observ the serum isozymes of Lactate dehydrogenase(LDH) by polyacrylamide gel eldctrophoresis ,5 bands of LDH isozymes(LDH 1～LDH 5)were observed on the gels,and it was discovered that there exist three genetic variants (A、B and C)in H subunit of LDH,the C variant took 82.35 percent of the total.Also,the relative activity of LDH 2 in the serum were significantly lower with A and B type of H subunit than that with C type in Jiali region , Its mechanism and significance remains further investigation. \ \