实验感染山羊关节炎—脑炎病毒的绵羊抗体应答反应的研究

Studies on the antibody response of sheep experimentally infected with caprine arthritis encephalitis virus

本实验用琼脂凝胶免疫扩散试验和免疫印迹试验对实验感染山羊关节炎—脑炎病毒（ＣＡＥＶ）的绵羊抗体应答反应进行了研究，用两种方法都可在接毒绵羊的血清中检测到ＣＡＥＶ的抗体。琼脂凝胶免疫扩散试验最早可于接毒后的第７周时检测到抗体，免疫印迹试验最早可于接毒后的第６周时检测到抗ＣＡＥＶ的ｇｐ１２５、ｇｐ４４、ｐ３５、ｐ２８和ｐ１４的抗体，这说明免疫印迹试验更为敏感一些。本实验的结果表明ＣＡＥＶ可在绵羊体内诱生明显的体液免疫应答反应，因此用ＣＡＥＶ通过绵羊体传代的方法可能会得到具有良好的抗原性的ＣＡＥＶ毒株，这对于人工培养ＣＡＥＶ强毒是非常重要的。此外。

In this experiment the humoral antibody response of sheep experimentally infected with caprine arthritis encephalitis virus (CAEV) was analyzed utilizing agar gel immunodiffusion test (AGIDT)and immunoblot assay (IBA).Antibody against CAEV antigen was detected in sera of sheep inoculated with CAEV by AGIDT and IBA.The initial detection of antibody against CAEV antigen was the 7th week after inoculation by AGIDT but it was the 6th week after inoculation by IBA and antibodies against gp125,gp44,p35,p28 and p14 of CAEV were detected in sera of sheep inoculated with CAEV,indicating that IBA is more sensitive than AGIDT.The results of this experiment showed that obvious humoral immune response was induced in sheep inoculated with CAEV,so it is possible to obtain a CAEV strain with good antigenicity by passages of CAEV in sheep.This is very important to artificial cultivation of a virulent CAEV.On the other hand,this experiment also provides very practical detection methods for passages of CAEV in sheep.