超临界CO_2流体提取车前子总黄酮的工艺优化

The CO_2 Supercritical Fluid Extraction Technology of Flavonoids in Semen Plantaginis

通过正交实验研究,对超临界CO2流体提取车前子中黄酮类化合物的工艺进行了优化,实验结果表明各因素对总黄酮提取率的影响程度由小到大依次为:提取温度、夹带剂用量、提取压力、提取次数。在提取时间为0.5 h的条件下,最佳提取工艺条件为:提取温度45℃、压力300 Bar、夹带剂无水乙醇用量2.5 mL/g原料、提取2次。

The CO2 supercritical fluid extraction technology of flavonoids in Semen Plantaginis was optimized by orthogonal experiments. The experimental results showed that the order of different factors affecting extraction rate was extraction time 〉 extraction pressure 〉 ethanol dosage 〉 extraction temperature. At the condition of 0.5 h extraction time, the optimal experimental parameters were 45 ℃ extraction temperature ,300 Bar extraction pressure,2.5 mL/ （g materials） ethanol dosage, twice. The results showed that the extraction rate of flavonoids was considerable.