^(99m)Tc-DTPA-DG对乳腺癌MCF-7细胞增殖的影响

Effects of ^(99m)Tc-diethylenetriamine pentaaceitc acid-glucosamine on proliferation of breast cancer cells

目的观察99mTc-DTPA-DG对人乳腺癌MCF-7细胞DNA合成的影响,探讨99mTc-DTPA-DG作为一种分子显像剂评价肿瘤的疗效。方法采用一步法合成99mTc-DTPA-DG,培养人乳腺癌MCF-7细胞,分别加入不同浓度的DTPA-DG、FDG和DG(每毫升生理盐水中分别加入DTPA-DG、FDG和DG为5mg,25mg及50mg),干预24h后,掺入3H-TdR6h,检测3种药物是否参与人乳腺癌MCF-7细胞DNA的合成,比较不同的浓度下细胞的摄取率,以及不同药物对细胞增殖的影响。结果DTPA-DG及DG均掺入人乳腺癌MCF-7细胞的增殖,DTPA-DG组摄取率高于DG组和FDG组(n=4,P<0.00),且浓度为0.5mg时3H-TdR摄取率最高。FDG随着时间的延长和浓度的升高,摄取率逐渐下降,可能不掺入细胞核或者产生了细胞毒性。结论99mTc-DTPA-DG能够参与肿瘤细胞的增殖,反映细胞核的活性,可以作为一种分子显像剂评价肿瘤的疗效。

[Objective] This study was to evaluate the effect of ^99mTc-diethylenetriamine pentaaceitc acid-glucosamine （^99mTc-DTPA-DG） on the DNA cloning of breast cancer cells MCF, and approach their potential value to cancer diagnosis and therapeutics as molecular imaging modalities. [Method]^99mTc-DTPA-DG was synthesized and labeled by stannous chloride reduction method. To evaluate whether ^99mTc-DTPA-DG is involved in cell nuclei activity, in vitro thymidine incorporation were conducted using breast cancer cells. Human breast tumor cells were plated at 5×10^4 cells/well, DTPA-DG, FDG and DG （0.1mg/20μL, 0.5 mg/20μL, 1.0 mg/20μL） and 20μL saline （control） were added to this wells. After 24 hours, each well was applied with 0.5μCi/10μL saline of ^3H-TdR and incubated for 6 hours. Finally, cells were added with the cocktail, and radioactivity was counted. Then detect wether three kinds of drugs are involved in cell nuclei DNA synthesis and compare to uptake ratios in different concentration. [Results] [^3H] Thymidine incorporation assays indicated that DTPA-DG and DG incorporated into cell nuclei activity. The uptake ratios for DTPA-DG group is higher than DG and FDG （P 〈0.00）. The maximal uptake ratio occured concentration of 0.5 rag. FDG had less involvement in cell nuclei activity. [Conclusion] ^99mTc-DTPA-DG is involved in cell nuclei activity and coulg assess the therapeutic tumor response.