一种筛选拟南芥低铁响应突变体的有效方法

An Efficient Method for Screening Mutants With Altered Responses to Iron Deficiency in Arabidopsis

铁是植物必需的一种微量营养元素。农作物的产量及品质经常因土壤缺铁而降低。研究植物在低铁条件下生长发育及对铁缺乏响应的分子机制,有助于揭示植物铁营养的遗传基础,有利于改良和培育耐低铁的农作物。笔者根据野生型拟南芥在低铁条件下的生长和生理表型确定了遗传筛选突变体的指标,并建立了筛选拟南芥与缺铁信号及信号转导相关突变体的方法。利用这种方法,笔者筛选出11株突变体并克隆了一个可能与植物耐低铁胁迫相关的基因,证明该方法适用于鉴别铁营养相关的重要基因以及分子遗传学的研究。

Fe is an essential mineral nutrient for plant growth and development. Iron deficiency directly affects crop yield and quality. However, the molecular mechanism of iron nutrition is largely unknown. To understand how plant response to Fe deficiency stress, in this paper, we have developed an efficient method for genetic screening Arabidopsis mutants related to the stress based on the phenotypes in morphology and physiology. Using this screening method, we have isolated 11 putative T-DNA tagged mutants with alterations in sensitivity in response to iron deficiency. We have cloned one gene, which mutation caused increased sensitivity to Fe deficiency. Identification and characterization the determinants in Fe nutrition of plant will further our understanding on genetic basis of plant in response to nutrient stress. It will provide new insights on molecular improvement of crops.