水生美人蕉组培快繁技术研究

Research on Tissue Culture and Rapid Propagation of Canna glauca L.

以水生美人蕉带有芽苞的根茎为外植体进行组培快繁技术研究,结果表明:MS+BA1.0～4.0mg/L+NAA0.2mg/L培养基能较好地诱导分化出不定芽;MS+BA6.0～8.0mg/L+NAA0.2mg/L培养基增殖效果最好;在MS不加激素和MS+NAA0.2mg/L培养基上进行生根培养都有不同程度的根分化。株高6～8cm出瓶用苔藓炼苗,成活率达95%以上。

This article mainly studied on the fast propagation technology system of tissue according to Canna g/auca L. with buds as explant. The result show that： MS＋BA1.0-4.0mg/L medium can induce movable bud better than general methods, MS＋BA6.0-8.0mg/L medium has the best effect in reproducing aspect, there both have different degree ＋NAA0.2mg/L culture ＋NAA0.2mg/L culture root detach when using culture medium MS （not include hormone） and MS＋NAA0.2mg/L culture medium culture root. Young plant can come out bettle when its height reaches to 6-8cm. Moss can be used to exercise young plant. The survive ratio comes to 95%.