草莓乙烯受体FaEtr2基因的克隆及其反义表达载体的构建

Cloning of Ethylene Receptor Gene of FaEtr2 of Strawberry and Construction of the Plant Antisense Expression Vector

为了构建草莓乙烯受体FaEtr2基因的反义表达载体,在已报道的FaEtr2基因序列的基础上,设计特异引物,克隆草莓乙烯受体FaEtr2基因部分特异序列,将该片段反向插入植物表达载体pBI121的CaMV 35S启动子和NOS终止子之间,构建了反义表达载体pBI121Etr2。通过双酶切鉴定后,导入农杆菌EHA105中,酶切和PCR鉴定表明质粒已导入到农杆菌中。本研究为后期该反义基因转化草莓品种以改良草莓果实耐贮运性打下基础。

The objective is to construct of the plant antisense expression vector of ethylene receptor gene of FaEtr2of strawberry.Specific primer was designed according to the reported cDNA sequence of theFaEtr2, and partial sequence of FaEtr2 was obtained. FaEtr2 antisense sequence was then inserted between the CaMV 35S promoter and NOS terminator into the expression vector pBI121, The expression vector was called pBI121Etr2 .The expression vector was checked then with two enzymes cutting. Then made the antisense expression vector into Agrobacteriurn tumefaciens EHA 105. The conclusion was that the vector has been transformed into EHA 105, which make a found of further study on delay-ripening of strawberry.