超临界CO_2萃取胡萝卜中类胡萝卜素的研究

STUDY ON TECHNOLOGY OF SUPERCRITICAL CO_2 EXTRACTION OF CAROTENOID

采用超临界CO2萃取技术萃取胡萝卜中的类胡萝卜素。研究了萃取压力、温度、时间和携带剂对提取率的影响。确定工艺条件为:压力30MPa,萃取温度45℃,萃取时间3 h,携带剂为石油醚。用高效液相色谱对类胡萝卜素进行分析,结果表明:经萃取的类胡萝卜素样品与标准β-胡萝卜素样品的谱图特征峰基本一致。

Technology of supercritical CO2 extraction of carotenoid was adopted. The effects of pressure, temperature, time and entrainer were determined. The optimun conditions were pressure 300 Mpa, temperature 45 celsius degree , extraction time 3h and the entrainer was petroleum ether . On the anlysis of carotenoid by High Pressure Lipid Chomatography, the results show that the extracted carotenoids＇ chomatogram characteristic wavecrest were basically consistent with the standard samples.