5-Br-PADCAP直接光度法测定血清未饱和铁结合力

A New Analytical Method for UIBC in Serum:5-Br-PACDAP Direct Spectrophotometric Method

为建立简便、灵敏的双试剂自动化分析法测定血清未饱和铁结合力（UIBC），在表面活性剂存在下，用2-（5-溴-2-吡啶偶氮）-5-[（N，N-二羧基甲基）氨基]苯酚（5-Br-PADCAP）为显色剂测定血清UIBC。结果表明，线性范围0～90μmol/L，平均回收率101.57％，批内变异系数（CV）和批问变异系数分别为0.028、0.046，与日本和光UIBC测定试剂盒比较具有良好的相关性，线性回归方程和相关系数分别为Y=1.0133X-0.3356，r=0.9961。108例健康人血清UIBC含量为31.65～50.81μmol／L（χ±2s）。用5-Br-PADCAP双试剂自动化分析法测定血清UIBC方法简便、灵敏可靠，适合临床应用。

To establish a simple, sensitive automatic method of determination of UIBC in serum with two reagents. Serum UIBC was determined with 5-Br-PADCAP in the presence of surfactant. The method was liner in the range of 0～90μmol/L, the average rate of recovery was 101.57%, the withinrun and between run CV was 0.028 and 0.046, respectively. Comparing this method （у） with Wako UIBC reagents （х）, we obtained the regression equation, у=1.0133х-0.3356, г=0.9961. The reference interval （х±2s） was 31.65～50.81μmol/L （n=108）. This method is simple, sensitive and suitable for clinical application.