百合杂种胚的离体培养

HYBRID EMBRYO IN VITRO CULTURE IN LILY(Lilium L. )

以剥除胚乳的两个百合杂交组合的杂种胚，进行高体培养，5d后即开始萌发，15d后，萌发率达88％．在不同激素含量的培养基比较试验中，发现以MS＋NAA0．5mg／L＋KT1．0mg／L激素浓度培养基培养效果最好．其中，75％的种胚长成完整植株，12．5％的种胚长出幼芽，培养过程中，无根的幼苗生长至3cm左右，约5张叶片时，转移至MS＋NAA0.1mg／L的培养基上，2～3周后，有80％的幼苗长出根．而未利除胚乳的种子，45d后才观察到胚的萌发，68d后，仅16％的种子萌发．

Some of lily cultivars are important ornamental gardening plants,and rely on asexual reproduction to propagate themselves mostly. But the sexual cross is one of necessary means for breeding. We made some cross combination in Lilium L.,and the hybrid seeds of two cross combinations were used as materials for hybrid embryo in vitro cuIture. After surface sterilization, the seeds were inoculated on 1 % agar plates at high density, 2 days later, some of the seeds were laid on the MS, medium (without hormones )directly,and some of them were dissected,the endosperms were excised and the embryos were laid on the MS0medium. After 5 days, the embryos began to germinate and the germinating embryos amounted to 88% during 15 days,but the seeds whose endosperms were not excised began to germinate after 43 days, and the germinating seeds made up only 16% until 68 days later.Among the different media tested,MS supplemented with NAA 0. 5 mg/L and KT 1. 0 mg/L was the most effective for embryos germination, 75 % embryos grew into plantlets and 12. 5 % embryos grew into buds. When the buds were transferred on MS supplemented with NAA 0.1 mg/L medium,80% buds grew into plantlets after 4 weeks.