周期性牵张力调节人牙周膜细胞成骨相关基因表达的研究

Effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells

目的探讨牵张力诱导体外培养的人牙周膜细胞(human periodontal ligament cells,HPDLC)成骨分化的基因调控机制。方法通过体外细胞加载系统对培养的人牙周膜细胞施加12%形变率、6周/min 的周期性牵张力48h,使用点样数为96点的人骨再生基因表达谱芯片检测周期性牵张力加载组细胞成骨相关基因表达的变化。结果 HPDLC 在周期性牵张力作用下有21种成骨相关基因的表达明显升高,包括10种生长因子和相关分子,10种细胞外基质和相关蛋白,1种细胞黏附分子。有2种基因表达明显下降,包括1种生长因子和相关分子,1种细胞黏附分子。结论周期性牵张力作用于体外培养的 HPDLC,可以通过调节部分成骨相关基因的表达,诱导牙周膜细胞的成骨分化。

Objective To investigate the effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells （HPDLC）. Methods HPDLC were cultured on flexible-bottomed plates and subjected to 12% elongation by strain unit at 6 cycles/min （ i. e. 5-s elongation and 5-s relaxation） for 48 hours in the experimental groups. GEArray Q series Human Osteogenesis Gene Array was used to identify the genes expressed in HPDLC, including growth factors and associated molecules, extracellular matrix and its associated proteins, cell adhesion molecules and housekeeping genes. The changes in the expression of 96 representative transcripts were determined by arrayed cDNA hybridization. Results After application of tension force, 21 genes were significantly upregulated, including 10 growth factors and associated molecules, 10 extracellular matrix and its associated protein and 1 cell adhesion molecules. Two genes were significantly downregulated, including 1 growth factors and associated molecules and 1 cell adhesion molecules. Conclusions The HPDLC can differentiate into osteoblast-like cells by mechanical stretch induction through the activation of some osteogenesis genes.