枸头橙种子中柑桔衰退-茎陷点病毒的鉴定

INDEXING OF SEEDS OF GOUTOUCHENG SOUR ORANGE FOR CITRUS TRISTEZA STEM PITTING VIRUS

采用双抗体夹心－酶联免疫吸附测定试验（ＤＡＳ－ＥＬＩＳＡ）对枸头橙种子中柑桔衰退－茎陷点病毒的带毒情况进行了鉴定。结果表明，从严重感染茎陷点病的枸头橙罹病树采集的种子可检测到柑桔衰退病毒（ＣＴＶ）。种子带毒量以内种皮为较高，剥皮种子次之，外种皮呈阴性。种子在４℃冰箱内贮藏１～２年后仍带毒，但带毒量有所下降。对带毒种子繁殖的实生苗及嫁接在实生苗上的墨西哥来檬的检测结果，有部分呈阳性，种植在防虫隔离网室内的１００余株２年生枸头橙实生苗，用无毒珠心系的墨西哥来檬芽嫁接进行生物鉴定。

Indexing tests were done in seeds of Guotoucheng orange for citrus tristeza stem pitting virus with double antibody sandwish enzyme linked immunosorbent assay (DAS ELISA). The results showed that seeds collected from Guotoucheng orange seedling infected with stem pitting disease were positive for CTV SP. The consistency of CTV in inner seed coat was higher than that in peeled seed. It remained positive after one to two years′ storage of seeds in refrigerator at 4℃, but the consistency of CTV was reduced. External seed coat was negative. Young tissues collected from some of the seedlings propagated from infected seeds, as well as Mexican lime grafted on Guotoucheng orange seedlings were positive for CTV by DAS ELISA test. More than 100 of 2 years old Guotoucheng orange seedlings planted in insect proof screenhouse were grafted with buds of healthy Mexican lime, and no symtoms were observed for two years.