血小板反应素-4基因A387P多态性与心肌梗死的相关性研究

Correlation of thrombospondin-4 A387P polymorphism with myocardial infarction

目的 研究中国华东地区汉族人群血小板反应素-4（thrombospondin-4,TSP-4）基因G29926C（A387P）多态性与心肌梗死（MI）发病的关联性.方法 应用聚合酶链反应一限制性片段长度多态性方法分析TSP-4 A387P多态性,将639例患者分为MI组和对照组,MI组为302例经冠状动脉造影确诊的MI患者,对照组为337例冠状动脉造影阴性、排除冠心病诊断者.结果 GC基因型在两组的分布,差异无显著性意义（5.3%νs7.1%,P=0.34）,未检测到CC纯合予.C等位基因频率在两组的分布,差异无显著性意义（2.6%νs 3.6%,P=0.35）,对照组TSP-4 387A→P变异频数明显低.多元logistic回归分析显示TSP-4 A387P多态性与MI的发生无显著性相关（OR=0.81,95%CI：0.41～1.62,P=0.55）.结论 TSP-4基因387A→P变异在中国华东地区汉族人群中发生频率明显低,且与MI的发病无显著相关性.

Objective To investigate the possible association between the thrombospondin-4 （TSP-4） gene G29926C （A387P） polymorphism and myocardial infarction （MI） in the Chinese Han population. Methods A case-control study was conducted in 302 patients with angiographically documented MI and 337 control subjects free from coronary artery disease and had normal coronary angiograms. The TSP-4 A387P polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis. Results The frequencies of GC genotype were 5.3% and 7.1% in MI group and control group （ P=0.34）. No homozygote of the C allele was detected. The prevalence of the C allele was not significantly different between patients with MI and controls （2.6% vs 3.6%, P =0.35）. The prevalence of the C allele in control subjects was lower. Multivariate logistic regression analysis indicated that there was no significant correlation between TSP-4 A387P polymorphism and MI （ OR=0.81, 95% CI=0. 41-1.62, P=0.55）. Conclusion The TSP-4 A387P variation shows a dramatically lower prevalence in the Chinese Han population and has no significant correlation with risk of MI.