摘要
在ＳＰＦ来源的一日龄雏鸡，不论是注射能表达马立克病病毒（ＭＤＶ）的３８ｋｄ磷蛋白（ｐｐ３８）基因的重组杆状病毒感染的昆虫Ｓｆ９细胞裂解物还是注射经亲和层析提纯的重组ＭＤＶｐｐ３８，都能抑制雏鸡的体液免疫反应，表现为相应雏鸡对注射小鼠红血球５～７ｄ后的血球凝集抗体的平均滴度比对照鸡低１．３８～１．６８个Ｌｏｇ２（ｐ＜０．０２５）和２．５５～３．２５个Ｌｏｇ２（ｐ＜０．００５）。
In chicks of SPF origin injected with Marek’s disease virus (MDV) 38 kd phosphorylated protein (pp38) expressing recombinant baculovirus infected insect Sf9 cell lysates or the recombinant pp38 purified from the infected Sf9 cell lysates through affinity column,the humoral immunne response was depressed,and the average antibody titers of heamoglutination to mouse red blood cells in recombinant pp38 treated chicks were 1 38～1 68 Log2 (p<0 025) and 2 55～3 25 Log2 (p<0 005) lower than that in control chicks in 5～7 days after immunization with mouse red blood cells This is the first report of studies on the biological activities of MDV pp38
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第1期71-76,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目
关键词
马立克病毒
38kd磷蛋白
重组产物
雏鸡
免疫抑制
Marek’s disease virus (MDV),38 kd phosphorylated protein (pp38), Immunodepression
