HPLC法测定不同产地蜂胶中高良姜素的含量

Quantitative Analysis of Galangin from Propolis in Different Areas by HPLC

目的：建立蜂胶中高良姜素的含量测定方法。方法：采用HPLC法,Diamonsil（钻石）C18（46 mm×250mm,5μm）色谱柱,甲醇-0.4%的磷酸水（65∶35）为流动相,流速为1.0 ml/min,检测波长为256 nm。结果：高良姜素在0.00412-0.02472 mg/ml范围内呈良好的线性关系（r^2=0.9998）;高良姜素的含量,山东最高,为11.7 mg/g,其次是内蒙和河南,分别为10.5 mg/g,10.3 mg/g,甘肃最低为9.8 mg/g。结论：本方法简便、可靠,适用于峰胶中高良姜素的含量测定。

Objective： To establish an HPLC method for determination of galangin in propolis. Methods： HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution （65： 35） as mobile phase, at the flow rate of 1.0 mL/min and the UV detection wavelength at 256 nm. Results ： The linear of galangin was 0.00412 - 0. 02472 mg/ml（ r^2 = 0. 9998 ）. The content of galangin was the highest in the propolis from Shandong （ 11.7 mg/g）. The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g, 10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu （9. 8 mg/g）. Conclusion ： HPLC method is simple and reliable for determination of galangin in propolis.