Box-Behnken法优化提取霍山石斛活性多糖的研究

Studies on the Extraction of Active Polysaccharide from Dendrobium huoshanense with Box-Behnken Method

目的:为获得较高的多糖提取率,本文探讨了霍山石斛多糖的提取工艺及多糖脱色方法。方法:研究了多糖提取过程中影响浸提的几个因素。包括提取温度、提取时间、料液比、提取次数和提取pH,并通过Box-Behnken法优化了工艺条件,建立了工艺模型。结果:当提取温度为69.4℃,pH值为5.9,料液比为1∶20,提取次数为2次,提取时间为1.57 h时,霍山石斛多糖提取率最高,达到0.1821 g/g。DEAE-52脱色法效果较佳,色素脱除率达到65.47%。结论:采用Box-Behnken法建立的多糖提取工艺模型能很好地预测实验结果,偏差1.6%。通过模型方程求得最佳工艺条件,多糖提取率高,脱色效果好。

Objective： In order to obtain preferable extraction rate of polysaccharide from Dendrobium huoshanense, best extraction conditions and decoloration method were investigated. Methods： Five influential factors including extraction temperature, extraction time, ratio of material to water, repetition times and extraction pH were carried out. Extraction model of polysaccharide from Dendrobium huoshanense was established by Box-Behnken method. Results： In the conditions of 69.4℃ of the temperature, 1.57h of the extraction time, 1 ： 20 of the ratio of material to water, two of the repetition times and 5.9 of the pH, the extraction rate reached the peak of 0. 1821 g/g. Taking into account of decoloration, 65.47% of decoloration rate was obtained with DEAE-52 method. Conclusion： Extraction model of polysaccharide from Dendrobium huoshanense established by Box-Behnken method can precisely predict the experiment results. In the best conditions deduced from the model, the extraction rate of polysccharide is high and so is the decoloraion rate.