均匀设计法优选甘草的半仿生提取工艺条件

Optimizing the Best Extraction Condition of Semi-bionic Extraction for Licorice by Unifrom Design

目的:优选甘草的半仿生提取的最佳工艺条件。方法:以甘草酸含量、总黄酮含量、总多糖含量、HPLC总面积、浸膏得率等为指标,采用均匀设计优选其半仿生提取法(简称SBE法)的条件。结果:优化条件为:三煎用水的PH值依次为5.88,7.50,8.90;煎煮总时间为3.93 h。结论:结合工业生产实际,确定其SBE法工艺条件为:三煎用水的PH值依次为6.0,7.5,9.0;煎煮时间依次为2,1,1 h。

Objective ： To optimize the best extraction condition of semi-bionic extraction for licorice. Methods ： The best extraction condition of the semi-bionic extraction for licorice by uniform design was optimized with glycyrrhizic acid, total flavonoids, total polysaccharides, total HPLC integrate area and dried extract weight as the indexes. Results： The optimized technologic condition： The pH values of the water for the 1st, 2nd, and 3rd decoctions were in order of 5.88,7. 50, and 8. 90, and the total extraction time was 3.93 h. Conclusion ： According to industry production condition, the best SBE technologic condition is ： The pH values of the water for the 3 decoctions are in order of 6.0, 7.5, and 9.0, and the extraction time are 2h, lh, and lh, respectively.