CDA-Ⅱ对乳腺癌细胞生长抑制作用的可逆性实验研究

Reversible Effect of Uroacitides on the Growth Inhibition of Breast Cancer Cells

目的研究癌细胞分化诱导剂尿多酸肽(CDA-Ⅱ)对乳腺癌细胞生长的抑制作用是否具有可逆性。方法将CDA-Ⅱ与乳腺癌细胞株MCF-7和MDA-MB-231进行体外培养,然后去除培养液中的CDA-Ⅱ,再观察其对乳腺癌细胞生长曲线、细胞周期及形态学等方面的影响。结果去除CDA-Ⅱ后,乳腺癌细胞的生长和增殖能力增加,乳腺癌细胞S期比例增加。结论CDA-Ⅱ对乳腺癌细胞生长和增殖的抑制能力具有可逆性。

Aim To investigate the reversible growth inhibition effect of CDA-Ⅱ on breast cancer cells. Methods The effect of CDA-Ⅱ on growth curve, cell cycle and morphology of breast carcinoma cell lines MCF-7 and MDA-MB- 231 were observed in vitro cultures with CDA-Ⅱ and with depletion of CDA-Ⅱ in the culture medium. Results With depletion of CDA-Ⅱ in the culture medium, the growth speed, proliferation ability and the percentage of phase s breast tumor cells were increased. Conclusion The growth inhibition effect of CDA-Ⅱ on breast carcinoma is reversible.