应用比较基因组杂交技术研究外阴鳞癌组织的遗传变异

Chromosomal Aberration in Vulvar Squamous Cell Carcinoma Analyzed by Comparative Genomic Hybridization

背景与目的:外阴鳞状细胞癌约占女性外阴恶性肿瘤的80%～90%。但有关其遗传学研究却不多,本研究采用比较基因组杂交(comparative genomic hybridization,CGH)技术分析外阴鳞癌组织的遗传变异,了解其遗传改变的特征。以期发现外阴鳞癌相关基因。方法:应用CGH技术检测21例外阴鳞癌基因组的不平衡性即其DNA的扩增或丢失。结果:外阴鳞癌常见的扩增染色体是3q,8q,12q。常见的丢失染色体是4p和3p。结论:外阴鳞癌细胞中存在多条染色体拷贝数的改变,由此引起相应癌基因的扩增和抑癌基因的丢失可能参与了外阴鳞癌的发生、发展。

BACKGROUND ＆ OBJECTIVE： Vulvar squamous cell carcinoma （VSCC） accounts for about 80%-90% of female vulvar malignant tumors, but the etiology is still unclear. This study was to identify genetic alteration in VSCC by comparative genomic hybridization （CGH）. METHODS; The genomic imbalance, that is, gains or losses of chromosomes, in 21 cases of vulvar squamous cell carcinoma was detected by CGH, RESULTS： The common chromosomal alterations in VSCC included gains of chromosomes 3q, 8q and 12q, and losses of chromosomes 4p and 3p. CONCLUSIONS; There are multiple chromosomal aberrations in VSCC. The amplification of the tumor suppressor genes and loss of the oncogenes on these regions may be involved in the development and progression of VSCC.