摘要
以大豆分离蛋白(SPI)、乳糖及可溶性淀粉(SS)为主要原料,利用微波辐射的方法合成SPI-糖接枝物,并对其相对分子质量进行了研究。经Sepharose CL-6B柱层析分析,SPI-乳糖接枝物相对分子质量(RMW)相对于SPI变化不大,而SPI-SS接枝物未检测到RMW大于440000的组分。高效液相色谱(HPLC)分析显示,SPI-糖接枝物RMW在10000~80000的含量明显高于SPI。
The soy protein isolate (SPI), lactose and soluble starch (SS) were used as the main materials, SPI-saccharide grafts were synthesized by microwave radiation and the changes of their relative molecular weight were also studied. Compared with SPI, relative molecular weight(RMW) of the SPI-lactose graft was not changed by gel chromatography in Sepharose CL-6B, and the component of RMW beyond 440 000 was not detected in the SPI-SS graft. Moreover, HPLC analysis showed that the component of RMW between 10 000 and 80 000 in SPI-saccharid grafts was higher than that in SPI.
出处
《中国油脂》
CAS
CSCD
北大核心
2007年第6期19-23,共5页
China Oils and Fats
基金
国家自然科学基金(20374025)
河南工业大学引进人才专项基金(150220)
关键词
大豆分离蛋白-糖接枝物
微波
相对分子质量
soy protein isolate-saccharide grafts
microwave
relative molecular weight