冷冻对同种异体鼠坐骨神经移植免疫反应的影响

Affection of Cryotherapy for Immunological Reaction of Allograft of Rat Sciatic Nerve

目的:探讨应用冷冻法与免疫抑制剂法对同种异体鼠坐骨神经再生效果的比较。方法:实验分为新鲜自体神经移植组,新鲜异体神经移植组。供体神经冷冻后异体神经移植组.异体神经移植后应用免疫抑制剂组。于移植后3周各组分别进行电生理学、组织学、电镜、混合淋巴细胞培养(MLC)及迟发性超敏反应(DTH)检查神经移植效果。结果:各组神经移植效果由优至差排序为:新鲜自体神经移植组,供体神经冷冻后异体神经移植组,应用免疫抑制剂组.新鲜异体神经移植组。结论:周围神经的细胞成分可被低温保存,移植后可存活并具有功能,冷冻法优于应用免疫抑制剂法。

Objective：To discuss the comparison on effection between cryotherapy and immunosuppressive agent for allograft of rat sciatic nerve. Methods：The experiment animals were divided into fresh autograft group, fresh allograft group, autograft group of freezing donor nerve and allograft group applied with immunosuppressive agent. The electric physiology, histology, electromicroscope mixed lymphocyte culture and delayed super-allergy reaction of transplantation were tested. Results：The effect of nerve transplantation sequenced from better to worse： fresh autograft group, autograft group of freezing donor nerve, allograft group applied with immunosuppressive agent, fresh allograft group. Conclusion： The cellular component of peripheral nerve could be preserved with low temperature. Cryotherapy could decrease lymphocyte immigrating to transplanted tissue.The effect of cryotherapy transplantation might be better than that of immunosuppressive agent group.