多发性肌炎的动物模型及雷公藤多甙对其疗效的研究

Polymyositis animal model and glucosidorum triptery giitotorum therapy of it.

目的 寻找一种多发性肌炎的良好动物模型及其有效的治疗药物。 方法 给动物注射同种大鼠的肌匀浆 ,观察其活动状态 ;通过肌肉组织染色及ELISA法 ,研究肌肉的病理学变化 ,同时给动物喂服雷公藤多甙观察药物疗效。 结果 部分动物表现肌无力和肌萎缩 ,大多数肌肉组织学检查示肌纤维炎性变 ;免疫组化检查可见肌膜、肌内膜和肌束膜中有IgG沉积。动物服雷公藤多甙后肌肉病变明显减轻 ,其疗效与强的松相似。 结论 用同种大鼠肌匀浆免疫动物可诱导出多发性肌炎的动物模型 ;雷公藤多甙能有效地治疗多发性肌炎。

Objective To investigate a good animal model and effective medicine for polymyositis, provide an ideal experimental base for studying its pathogenesis and theraputic methods. Methods The tested animals were injected intramuscularly homologous crude skeletal muscle homogenate, to observe the myopathological changes by using muscle histological stains and ELISA. The effect of glucosidorum tripterygiitotorum on the myopathological changes was observed.Results Some rats manifested limb muscle weakness and atrophy. Major animals showed inflammatory myopathological changes. There were depositions of immunoglobulin in sarcolemma, endomysium and perimysium. The myopathological changes were improved after feeding glucosidorum tripterygiitotorum to the animals and there were no significant differences between the effect of glucosidorum tripterygiitotorum and prednisone. Conclusions Experimental polymyositis model of rats can be induced by homologous skeletal muscle homogenate. Glucosidorum tripterygiitotorum is an effective medicine for polymyositis.