Zn胁迫对黑麦草幼苗生长、生理生化及Zn吸收的影响

Effects of Zinc stress on growth,physiological and biochemical and Zn uptake of Ryegrass(Lolium perenne L.)

采用营养液培养法,研究了不同Zn浓度(0,0.25,0.50,1.00,2.00 mm o l/L)对黑麦草幼苗生长、过氧化物酶活性、脯氨酸、根系活力及Zn吸收的影响。结果表明,低锌胁迫对黑麦草幼苗生长无抑制,过度锌胁迫(Zn≥2 mm o l/L)将降低黑麦草地上部干质量。幼苗叶内游离脯氨酸含量随锌胁迫时间、锌浓度增加而增加。随Zn胁迫时间增加幼苗POD活性先降后升、根系活力先升后降,锌处理的植株地上部POD活性随锌浓度增加先降低,然后增加,而根系活力随锌浓度增加而增加。黑麦草幼苗地上部和根系Zn含量随Zn浓度的增加而增加,当Zn浓度为2.00 mm o l/L时,地上部Zn含量最大值为775.0 m g/kg。

Solution culture experiment was carried out to study the growth, activity of preoxiodse （POD）, proline content, root system vigor, and Zn uptake under the different Zinc levels（0, 0. 25, 0. 50, 1.00, 2. 00 mmol/L） in ryegrass （Lolium perenne L. ）. The results showed that low Zinc stress did not inhibit the growth of plants, but the shoot dry weights decreased under excessive Zinc stress（Zn≥2 mmol/L）. The free proline content in shoot increased with the increase of time and Zinc level. The changes of POD activity and root system vigor were the parabola trend with the increase of time. The activity of POD in shoot decreased first, and then increased with the increase of Zinc level under Zinc treatment, while the root system vigor increased with the increase of Zinc level. The Zn content in the shoot and root increased with the increase of Zn level. The maximum of shoot Zn contents was 775.0 mg/kg（at 2.0 mmol/L Zn treatment）.