孕妇外周血中胎儿有核红细胞获取及鉴定的研究进展

Research Advance of Obtaining and Identifying Fetal Nucleated Red Blood Cells in Maternal Peripheral Blood

从孕妇外周血中获取胎儿有核红细胞是安全、方便,易于被孕妇接受的无创性产前诊断。提取孕妇外周血中胎儿有核红细胞最理想的方法是首先富集孕妇外周血,用胚胎或者胎儿血红蛋白抗体进行标记、识别,应用显微操作技术得到单个有核红细胞,继而扩增单个有核红细胞的基因组DNA,并进一步应用STR连锁分析验证为胎源性,进行遗传病诊断。

Obtaining fetal nucleated red blood cell（NRBC） from peripheral blood of pregnant women is a safe and convenient approach of non-invasive prenatal diagnosis, which is easily accepted. The idealest method of obtaining it is described as follow. NRBCs were separated from maternal peripheral blood, labled and identified with antibodies against embryo or fetal cells. Single NRBC was then collected by micromanipulator under microscopic observation followed by the whole genome amplification of single cell. After this, STR linkage analysis was used to verify the fetal origin for further diagnosis of hereditary disease.