SRAP分子标记分析西瓜遗传多态性

Genetic Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Citrullus lanatus

目的：探讨西瓜遗传多样性和遗传基础。方法：采用SRAP分子标记对西瓜品种D1、D2、D3、H1、H2、H3、M1、M2、M3、m1、m2、m3的多态性进行了分析。结果：每对引物组合产生13~25对比较清晰的扩增带.8对引物组合共产生131条扩增带。平均每对引物组合产生16.375条。8对引物组合共产生多态性带37条,每对引物组合产生3~7条,平均4.625条。每对引物组合产生的多态性带的比例为16.666%~38.464%,平均为28.675%。另外,对银染过程进行了优化。结论：SRAP标记多态性还是较高的,可以适于分析西瓜等遗传差异小的作物。

Objective：In order to investigate the Genetic diversity and genetic basis of Citrullus lanatus. Methods： Polymorphism among D1 ,D2, D3,H1 ,H2,H3 ,Mi ,M2 ,M3,m1 ,m2,m3 were analyzed with SRAP markers. Results：There were 13- 25 clear combination bands per primer. About 131 amplified fragments and 37 polymorphic fragments were detected 8 SRAP primer combinations. There were 3 - 7 clear polymorphic combination bands per primer. The number of amplified fragments and polymorphic fragments per primer combination were 16.375 and 4.625 respectively.The percentage of polymorphic fragments was 16.666% - 38.464%, average 28.675%. In addtion, opted the reaction of silver dyeing.Conclusion：All these results showed that the Polymorphism of SRAP markers is highely, and could be widely used in analyzed crops such as citrullus lanatus which has small Genetic Difference.