小牛血清去蛋白注射液对大鼠脑缺血早期脑组织中NO含量的影响

Effects of Deproteinised Calf Blood Serum Injection on NO and nNOS Expression at Earlier Period after Cerebral Ischemia Injury of Rats

目的探讨脑缺血早期,小牛血清去蛋白注射液通过影响脑组织中一氧化氮水平及一氧化氮合成酶的表达而产生的对神经元的干预作用及机制。方法动物分为假手术对照组、缺血模型对照组和小牛血清去蛋白注射液治疗组。局灶性脑缺血模型由线栓法阻塞大鼠大脑中动脉(MCAO)制成。在缺血4小时后进行相邻区域切片的nNOS免疫组化及HE染色,测定脑组织含水量,脑组织匀浆中NO和nNOS的含量。结果小牛血清去蛋白注射液能明显降低脑组织缺血损害的范围;与模型组相比,脑缺血4小时后DCSI组的脑组织含水量明显降低,脑组织匀浆中NO及nNOS的含量都明显降低(P<0.01)。结论小牛血清去蛋白注射液有可能是通过降低脑组织中神经型一氧化氮合成酶的表达,从而降低脑组织中一氧化氮的含量,而产生对大鼠脑缺血早期的保护作用。

Objective This paper has observed the effects of Deproteinised Calf Blood Serum Injection （DCSI） on NO and neuronal nitric oxide synthase （nNOS） expression at the early stage after permanent focal cerebral isehemia in rats and its mechanism. Methods The rats were classified randonly into Sham - operation group, cerebral isehemia model group and corresponding isehemia group pretreated with DCSI. Focal cerebral isehemia was produced by permanent middle cerebral artery occlusion （MCAO） with nylon suture inserted through the internal carotid artery. Carrying out contiguous slices HE stain and nNOS SABC immunohistochemistry and measuring the brain water content, BRA NO and nNOS was assayed by 10% brain tissue homogenate at the same time of 4h after occlusion. Results The NO and nNOS levels in 10% BRA tissue homogenate and the brain water content were increased significantly in model group, the Sham- operation group and DCSI group （P 〈 0. 01 ） . The results indicated that DCSI could depress the expression of nNOS at the early stage of cerebral isehemia and suggested that DCSI may play a protective role for nervous tissue in isehemie rains. Conclusions DCSI significantly restrains the up-regulated activity of nNOS after focal cerebral isehemia to protect the cerebral isehemie lesion from the early stage following the onset of isehemia.