珍珠黄杨春季扦插生根性状差异及内源激素变化

Rooting characteristics and endogenous hormone levels in Buxus sinica var. parvifolia during spring cutting

为了研究珍珠黄杨Buxus sinicavar. parvifolia扦插生根的相关机制,通过酶联免疫吸附分析(ELISA)技术对春季扦插生根过程中各品种的吲哚乙酸(IAA),脱落酸(ABA),异戊烯基核苷(iPA)和赤霉素4(GA4)质量分数作进程研究,并对其生根性状结果作统计分析。研究发现:在愈合组织产生前后及根突出表皮时,IAA、ABA和GA4质量分数的变化曲线在不同品种处理之间存在差异,3号和5号品种在ABT6处理后扦插生根能力有了提高。结果表明,易生根品种的激素质量分数在生根关键时期更利于插穗愈合组织的产生以及根原基的形成与分化。

Buxus sinica var. parvifolia （littleaf box） is one of the woody evergreen plant species that has high commercial value in the floriculture market. Efforts to improve propagation success will be essential in development of the species for commercial production and in satisfy the market＇s needs. The purpose of this research is to study the rooting mechanism of Buxus sinica var. parvifolia cutting. Seven cultivars were chosen, including five cultivars of littleleaf box and two of Buxus sinica （Chinese box） to compare the rooting potential. Enzyme-linked immunosorbent assay （ELISA） technology was used to measure the levels of indoleacetic acid （ IAA）, abscisic acid （ABA）, isopentenyladenosine （iPA）, and gibberellin4 （GA4） in the rooting zones, and the rooting characters were also observed in spring stem cutting. The results showed that around the time of callus forming and the root projecting epidermis, the levels of IAA, ABA and GA4 were different in seven cultivars and in different treatments. And the rooting ability of cultivar No. 3 and No. 5 treated by ABT 6 were improved. The high levels of endogenous hormone in the easy-to-root cultivars＇ in crucial rooting period was beneficial to callus generation and the root anlage formation and differentiation. [Ch, 4 fig. 2 tab. 18 ref. ]