谷氨酸生产菌噬菌体理化特性的初步研究

STUDIES ON CHARACTERISTICS OF TWO CORYNEBACTERIUM PHAGES

从味精厂废发酵液中分离得到２株谷氨酸生产菌噬菌体３Ｃ１３和３Ｃ４１．提纯的噬菌体经ＳＤＳ－ＰＡＧＥ分析：３Ｃ１３由１条主带、至少８条次带组成，多肽分子量在２８８４０～９５０００道尔顿之间；３Ｃ４１呈３条主带、３条次带，多肽分子量在１７３７８～７９４３３道尔顿之间．对提纯的核酸进行酶解消化、分子展层等生化分析以及限制性内切酶试验，证明２株噬菌体的核酸均为线状双链ＤＮＡ．３Ｃ１３核酸分子量为２７．８１×１０６道尔顿；３Ｃ４１为２７．

Bacteriophages 3C 13 and 3C 41 were isolated respectively from two strains of Glutamate producing bacteria that had become contaminated during an industrial fermentation.SDS PAGE showed that 3C 13 had one major band and more than eight minor bands, molecular weight of its polypeptide ranging from 28 840 to 95 000 daltons; as 3C 41 had three major bands,three minor bands and molecular weight from 17 378 to 79 433 daltons. Their nucleic acids were tested to linear ds DNA and the molecular weight of DNA were about 27.81×10 6 and 27.49×10 6 daltons respectively.