红豆杉细胞悬浮培养及动力学研究

Taxus Cell Suspension Culture and Metabolic Kinetics

对红豆杉细胞在５Ｌ通气机械搅拌式反应器中的培养过程及动力学进行了研究．经２４ｄ培养，细胞生物量增加３倍，紫杉醇含量可达细胞干重的１．０３５×１０－３．建立了描述细胞生长过程的模型，采用分段多项式函数逼近关联ｕ与ｕｍ的函数Ｆ（ｓ），建立了描述产物合成的动力学方程，采用多项式函数逼近关联产物合成速率与细胞生长速率的函数。

The metabolic kinetics of the Taxus cell suspension culture in a bioreactor including the cell growth curve, the sugar con sumption curve, the [PO 3- 4] consumption curve, the pH curve and protein contents curve, and the taxol contents in cells curve have been investigated. A model with a new function for describing the cell growth is developed. Some parameters have been determined.