马协型水稻细胞质雄性不育系线粒体基因组的变异与功能的研究

The Mitochondria Genomic Mutation and Function in the Cytoplasmic Male Sterile Line of Maxie Rice

马协型水稻细胞质雄性不育系是近年培育并广泛应用的一种新型不育系。利用Southern blot、Northern blot和Blue-native PAGE电泳等技术对其线粒体基因组的变异和功能进行研究,分析其雄性不育的分子机理。发现其不育系线粒体基因组中除有一个正常的cox2基因外还存在一个多余的拷贝,且分别转录成不同的转录本。Blue-native PAGE电泳显示,不育系线粒体呼吸链复合物IV的活性明显低于保持系。

Maxie is a newly inbreeded cytoplasmic male sterile （CMS） systems in rice （Oryza sativa L. ）. Our experiments showed that there existed an extra copy of cox2 gene in the CMS line except for the normal one. The two copies transcribed into different transcripts. In-gel activity assay showed that the mitochondria complex Ⅳ presented significant reduction in activity compared with the fertile line.