子宫内膜异位症患者腹水中TNF-α、TNF-β的测定

Determination of tumor necrosis factor-alpha and tumor necrosis factor-beta in peritoneal fluid of patients with endometriosis

目的：了解子宫内膜异位症（内异症）患者腹水中肿瘤坏死因子-α（TNF-α）和肿瘤坏死因子-β（TNF-β）的水平。方法：应用ELISA法,检测42例内异症患者（Ⅰ-Ⅱ期22例、Ⅲ-Ⅳ期20例）腹水TNF-α、TNF-β的浓度,并与对照组40例非内异症患者进行比较,分析TNF-α、TNF-β的浓度与内异症的相关性。结果：内异症组患者腹水中TNF-α、TNF-β的浓度明显高于对照组（P〈0.01和P〈0.01）,特别是Ⅲ、Ⅳ期比Ⅰ、Ⅱ期增高更明显（P〈0.01）,且两者的浓度呈正相关,相关系数为0.963。结论：内异症患者腹水中TNF-α、TNF-β明显增高,且与疾病严重程度相关。

Objective： To observe the levels of tumor necrosis factor - alpha （ TNF - α） and tumor necrosis factor - beta （TNF - β） in peritoneal fluid of patients with endometriosis. Methods： The concentrations of TNF - α and TNF - β in peritoneal fluid were measured by ELISA in 42 patients with endometriosis （22 with stage Ⅰ - Ⅱ and 22 with stage Ⅲ - Ⅳ ） and 40 women without endometriosis. Results. The concentrations of TNF - α and TNF - β in endometriosis group were significantly higher than those in control group （P 〈0. 01 ）. The levels of TNF - α and TNF - β in stage Ⅲand Ⅳ were significantly higher than those in stage Ⅰ and Ⅱ （P 〈0. 01 ）. Moreover, a positive correlation was found between the TNF - α and TNF - β levels. Conclusion ： Levels of TNF - α and TNF - β is not only, improved in endometriosis but also correlated with severity of disease, and they may be related factors inducing endometriosis.