HCV抗体阳性者血清B族载脂蛋白中HCV-RNA表达及与脂质的关系

Association of HCV-RNA expression with serum apolipoprotein B in patients with positive HCV antibody

目的:探讨慢性丙型肝炎患者HCV感染与脂类代谢的关系及特点。方法:采用免疫沉淀法,以羊抗人载脂蛋白B(Apo-B)抗血清沉淀HCV抗体阳性者血清Apo-B,再以PCR技术进行扩增检测Apo-B中HCV-RNA,并设多组阴性对照。结果:实验组15例HCV抗体阳性者血清Apo-B中,14例有HCV-RNA表达,且载量较高,而对照组26例血清及其Apo-B中却没有HCV-RNA表达。结论:HCV抗体阳性者血清Apo-B中检出HCV-RNA,进一步证实了HCV感染与脂质密切相关。

Objective： To discuss the relation between HCV infection and lipid metabolism in patients with chronic hepatitis C. Methods： Immunoprecipitation was adopted to precipitate serum apolipoprotein B （ Apo - B） by using sheep anti - human IgG in patients with positive HCV antibody. The HCV - RNA in Apo - B was determined with PCR amplification. Results： High HCV - RNA expression in Apo - B was detected in 14 of 15 patients with positive HCV antibody, however, the HCV -RNA expression was negative in both serum and Apo- B in 26 healthy individuals. Conclusion： HCV - RNA in serum Apo - B can be detected in patients with positive HCV antibody, indicating that HCV infection may be related with lipid metabolism.