酪氨酸激酶抑制剂抑制骨髓瘤细胞的增殖及Rac1的表达

Tyrosine kinase inhibitor inhibits multiple myeloma cell proliferation and the expression of Rac1 mRNA

目的:观察酪氨酸激酶抑制剂STI571对骨髓瘤细胞系RPMI8226细胞增殖、细胞周期以及Rac1 mRNA表达的影响。方法:采用MTT法检测STI571对骨髓瘤细胞增殖的影响;流式细胞仪检测对细胞周期的影响;半定量RT-PCR研究Rac1mRNA水平的变化。结果:STI571明显抑制RPMI8226细胞的增殖,24、48、72 h IC50值分别为(34.52±2.31)μmol/L、(28.46±1.58)μmol/L、(25.74±2.44)μmol/L。25μmol/L STI571处理24 h,G1期细胞由55.8%增加至72.4%,S期细胞由34.8%减至15.6%。半定量RT-PCR显示,Rac1 mRNA水平在25μmol/L STI571处理24 h后明显下降。结论:STI571能抑制RPMI8226细胞的增殖,并可下调其Rac1 mRNA水平。

Objective：To investigate the effects of tyrosine - kinase inhibitor STI571 on the RPMI8226 cell proliferation,cell cycle and the Rac1 mRNA expression. Methods： By means of MTT assays, the proliferation of RPMI8226 cells was determined. Flow cytometry was used to observe cell cycle. Rac1 mRNA level in RPMI8226 cells was examined by semi - quantitative RT - PCR. Results： The proliferation of RPMI8226 cells was significantly inhibited by STL571. After culture with STI571 for 24, 48, 72 hours, the cell mean IC50 values were 34.52 ± 2.31 μmol/L, 28.46 ± 1.58 μmol/L, and 25.74 ± 2.44 μmol/L . respectively. After administrated 25 μmol/L STI571 for 24 hours, RPMI8226 cells increased from 55.8% to 72.4% in G1 phase and decreased from 34.8% to 15. 6% in S phase. RT- PCR showed that the relative Rac1 mRNA level （Rac1/GAPDH） in RPMI8226 cells was downregulated following being treated with 25 μmol/L STI571 for 24 hours. Conclusion： STI571 can inhibit the proliferation of RPMI8226 cells, and downregulate Rac1 mRNA level.