应用RAPD方法分析厌氧氨氧化污泥驯化过程中的微生物遗传性质

Application of RAPD to analyze the genetic character of microorganisms during domestication of anaerobic ammonium oxidation sludges

采用随机扩增多态性DNA(RAPD)方法研究了厌氧氨氧化污泥驯化过程中微生物遗传多样性的变化,并对接种物不同的3个反应器中的微生物作了聚类分析.在污泥驯化培养过程中,3个反应器内的微生物发生了较明显的遗传变异,以缺氧污泥接种的反应器中微生物在驯化过程中的Nei基因多样性指数和Shannon信息指数均较高,遗传变异较大.硝化污泥中存在与厌氧氨氧化细菌亲缘关系较近的菌种,更适宜作为接种物驯化培养厌氧氨氧化细菌.以好氧污泥作为种泥启动反应器,通过培养硝化污泥再转入厌氧氨氧化驯化,这种驯化途径优于以缺氧污泥和厌氧污泥启动反应器的途径.

The genetic diversity of microorganisms present during the domestication of Anaerobic Ammonium Oxidation （ANAMMOX） sludges, was measured using the random amplified polymorphic DNA （RAPD） method and clustering analysis. During domestication, Nei＇s gene diversity and Shannon＇s information index of microorganisms in the reactor inoculated with anoxic sludge were higher than aerobic and anaerobic sludges, The Anammox homologous series was more likely to exist in the nitrification sludge, and aerobic active sludge was the optimal inoculum for the domestication of Anammox bacteria.