pH值对东北林蛙卵的孵化率及蝌蚪生长发育的影响

Effect of pH on Hatching Rate and Tadpole Development of Rana dybowskii

为探索水域pH值对东北林蛙（Rana dybowskii）卵孵化和蝌蚪生长发育的影响,在水温21-23℃的实验室条件下,采用单因子试验方法,研究了水环境不同pH值对东北林蛙卵的孵化率、蝌蚪的日增重、变态率及变态幼蛙初始重的影响。结果表明,受精卵对pH值的适应范围为4-11,孵化率较高的pH值范围为7-9;pH值为6,7,8,9组中的蝌蚪成活率分别为47.95%、48.98%、35.64%和43.64%。在pH值为5.5,6.5,7.5组中,林蛙蝌蚪的变态率分别为94.00%、93.00%和82.00%;变态幼蛙初始重分别为（267.33±46.97）mg、（278.67±37.67）mg和（260.67±35.52）mg。

In order to investigate the effect of pH on egg hatching and tadpole development of Rana dybowskii under the house temperature of 21 -23 ℃ and by the means of one- factor experiment,hatching rate of eggs, live body weight of tadpoles, metamorphosis rate and the body weight of newborn tadpole were studied. The results showed that the proper pH for egg hatching was from 4 to 11 and pH from 7 - 9 caused higher hatching rates than the others. The survival rates of 47.95 %, 48.98%, 5.65 % and 43.64% were obtained in testing groups of pH of 6,7,8 and 9 respectively. In the testing groups of pH of 5.5, 6.5 and 7.5, the metamorphosis rates were obtained 94.00% ,93.00%and 82.00% respectively, and the body weight of young frog after metamorphosis were （ 267.33 ±46.97） mg, （ 278.67 ± 37.67）mg, （260.67± 35.52）mg.