Ad-METH1对培养的内皮细胞分泌活性的影响

The effect of Ad-METH1 on secretion of ET-1 and bFGF from endotheliocyte

目的:研究基因重组血管抑制剂Ad-METH1(metalloprotease and thrombospondin1)对培养的内皮细胞分泌活性的影响,探讨血管生成抑制防治增生性瘢痕的可能机制。方法:制备重组血管抑制剂Ad-METH1,转染培养的人脐静脉内皮细胞,通过放射免疫分析法及酶联免疫分析法研究Ad-METH1对内皮细胞分泌内皮素-1(endothelin-1,ET-1)及碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的影响。结果:基因转染后24h内皮细胞ET-1、bFGF分泌受到明显抑制。结论:Ad-METH1对内皮细胞ET-1、bFGF分泌活性有影响,此是其早期参与抑制增生性瘢痕形成的可能机制之一。

Objective To investigate the effect of Ad-METH1 on endotheliocyte secretion activity and approach the possible mechanism of preventing hypertrophic scar by angiogenesis inhibition. Methods Ad-METH1 was prepared and used to transfect the cultural HUVEC. Then, the concentration of ET-1 and bFGF secreted from endotheliocyte in cultural supernate were studied by radioimmunoassay （RIA） and enzyme-linked immunosorbent assay （ELISA）. Results At the 24th hour after Ad-METH1 transfection, in experiment group, the concentration of ET-1 and bFGF in cultural supernate decreased markedly comparing to that in control group. Conclusion Inhibition of Ad-METH1 on secretion of ET-1 and bFGF from endotheliocyte might have act an important role in preventing the form of hypertrophic scar.