肝癌组织survivin的表达及其与HBV感染的关系

Expression of survivin protein in hepatocellular carcinoma tissues and its relationship with HBV infection

目的：研究肝癌组织中的survivin的表达与HBV感染的关系.方法：应用免疫组化法检测原发性肝癌组织45例,癌旁肝硬化组织25例,肝硬化组织18例,肝炎组织5例,正常肝组织10例中survivin的表达,同时检测原发性肝癌中HBsAg的表达.结果：在45例肝癌组织中survivin阳性34例（75.5%）,阳性率高于癌旁肝硬化组织（44.4%）,肝硬化组织（0）,肝炎组织（0）和正常肝组织（0,P〈0.001）.肝癌中survivin的表达在Edmondson分级Ⅲ~Ⅳ级高于Ⅰ~Ⅱ级（P=0.014）,而在年龄、性别、肿瘤大小、临床分期、是否有门脉转移方面无统计学差异（P〉0.05）,survivin与HBsAg的表达呈正相关（P=0.014）.结论：肝癌组织中survivin高表达说明其在肝癌发生发展中起重要作用,同时HBV感染对于sur-vivin表达上调有一定作用.

AIM ： To investigate the expression of survivin protein in human hepatocellular carcinoma （ HCC ） and its relationship with HBV infection. METHODS ： The expression of survivin in 45 cancer tissues, 25 adjacent cirrhosis tissues, 18 hepatic cirrhosis tissues, 5 hepatitis tissues and 10 normal liver tissues were assessed by immunohistochemical method and the expression of HBsAg in cancer tissues by the same method. RESULTS： Sur- vivin protein was expressed in 34 of the 45 cases of HCC （75.5%） and in 11 of the 25 cases of adjacent cirrhosis tissues （44.4%）. Expression of survivin protein was negative in 8 cases of hepatic cirrhosis, 5 cases of hepatitis and 10 normal liver tissues. Survivin protein positive expression rate in HCC was signiticanfly higher than that in hepatic cirrhosis, hepatitis tissues and normal tissues （ P 〈 0. 001 ）. Expression of survivln protein was significantly higher in those of Edmondson grade Ⅲ-Ⅳthan in those of grade Ⅰ-Ⅱ（ P = 0. 014 ） , but was not different in different age, sex, tumor size, clinical stage and local metastasis （ P 〉 0.05 ）. Expression of survivln protein was positively correlated with the expression of HBsAg （ P = 0. 014 ）. CONCLUSION： There is a marked increased survivin expression in HCC, which may play an important role in the formation of HCC. And HBV infection may has an effect on the up-regulation of the expression of survivin protein.