FHIT基因与HPV联合检测对宫颈癌诊断的意义

Significance of combined detection of FHIT gene methylation and high risk HPV for diagnosis of cervical cancer

目的通过检测宫颈癌患者DNA中脆性组氨酸三联体基因异常甲基化以及高危人乳头瘤病毒,以探讨二者联合检测在宫颈癌诊断中的意义。方法采用巢式甲基化特异性PCR方法检测21例宫颈癌患者脆性组氨酸三联体基因的甲基化状态,同时应用HC2法测定高危人乳头瘤病毒,并与32例宫颈上皮内瘤变患者和25例健康者作对照,分析二者联合应用的临床诊断价值。结果在13例宫颈癌患者标本检测到了脆性组氨酸三联体基因的甲基化,阳性率为66.7%,高危人乳头瘤病毒的阳性率为90.5%;平行联合检测时敏感性增至95.0%。宫颈上皮内瘤变组中脆性组氨酸三联体基因甲基化与高危人乳头瘤病毒阳性率分别为3.1%和28.1%;健康对照者标本中脆性组氨酸三联体基因无异常甲基化,高危人乳头瘤病毒阳性率为12.0%。结论联合检测患者脆性组氨酸三联体基因甲基化与高危人乳头瘤病毒可提高宫颈癌诊断的敏感性和特异性,对于宫颈癌的早期诊断具有积极意义。

Objective To investigate significance of combined detection of fragile histidine triad gene （FHIT） methylation in DNA of patients with cervical cancer and high risk HPV for diagnosis of cervical cancer. Methods In nested methylation specific polymerase chain reaction method, methylation status of fragile histidine triad gene（FHIT） of 21patients with cervical cancer and 32 patients with CIN and 25 healthy women was detected and their high risk HPV was detected in HC Ⅱ method. The results of the patients and normal women were compared in order to determine clinical diagnostic value of combined detection of FHIT gene and high risk HPV for cervical cancer. Results Aberrant promoter methylation of the FHIT gene was detected in 13 of 21 （66.7%） samples of patients with cervical cancer and the positive detectable rate was 66.7%. The detectable rate of high risk HPV was 90.5% （ 19/21 ）. Parallel combined detection increased the diagnostic sensitivity to 95.0%. The positive rates of FHIT gene methylation and high risk HPV of the patients with CIN were 3.1% and 28.1% , respectively. No aberrant methylation of the FHIT gene was found in 25 healthy women and their detectable rate of high risk HPV was 12.0%. Conclusion Combined detection of FHIT gene methylation and high risk HPV could increase sensitivity and specificity of diagnosis for cervical cancer, so it is of active significance for early diagnosis of cervical cancer.