ABC-ELISA定量测定红细胞趋化因子受体及其意义

Ouantitive Detection Erythrocyte Chemotatic Receptor (ECKR) with ABC-ELISA

目的建立ABC-ELISA定量测定红细胞趋化因子受体(ECKR)的方法并探讨其临床意义。方法分离一定数量红细胞与IL-8结合,离心后取上清液用ABC-ELISA法检测IL-8含量,通过公式计算红细胞趋化因子受体结合活性;对该法检测IL-8的灵敏度、精密度、准确度进行了探讨,并对31例正常人,25例恶性肿瘤患者和33例银屑病患者的ECKR进行了测定。结果该法检测IL-8的灵敏度为15.6 pg/mL。精密度批内平均CV 4.69%;批间平均CV 9.79%,准确度平均回收率为97%。31例正常人ECKR为(52.96±10.45)%;25例恶性肿瘤ECKR为(46.07±13.33)%;33例银屑病人ECKR为(46.87±10.28)%。经统计,恶性肿瘤患者和银屑病患者ECKR均比正常人ECKR要低(P<0.05)。结论该法是一种简便的定量测定ECKR的新方法,对研究红细胞免疫、趋化因子及其受体在疾病防治和发病机制中的作用有一定价值。

Objective To establish the detective method of quantitive ECKR with ABC- ELISA and discuss its clinical significance. Methods There were 31 cases of normal adult, 25 cases of malignant tumor and 33 cases of psoriasis patient who were detected IL- 8 with ABC - ELISA method in the ECKR activity and discussed the sensitivity, accuracy of the method. Results The sensitivity of IL - 8 detection was 15.6 pg/mL. The accuracy was CV4.69% in group and CV9.79% between groups. The recovery rate was 97%. The ECKR was 52.96- 10.45% in normal adult, 46.07 - 13.33% in malignant tumor group and 46.78 - 10.28% in psoriasis group that they were lower than that of nonnal group （P 〈0.05）. Conclusion This method might be a new raethod to study the RBC immunity, ECK and ECKR in value of prevention and its mechanism.