浮游微藻携带和传播WSSV的研究

Study on four phytoplanktons carried and spread WSSV

以对虾养殖池塘中常见的湛江等鞭金藻(Isochrysis zhanjiangensis)、亚心形扁藻(Platymonas subcordiformis)、盐藻(Dunaliella salina)和中肋骨条藻(Skeletonema costatum)四种浮游微藻为实验对象,研究其是否可以携带和传播严重危害对虾养殖业的对虾白斑病病毒(WSSV)。研究结果表明,这四种微藻和WSSV病毒粗提液混合后,经PCR检测发现在混合后96 h内微藻都可以携带WSSV,其中湛江等鞭金藻和亚心形扁藻在144 h内为WSSV阳性,盐藻在120 h内为阳性,然后在不同时间呈现WSSV阴性。采取病毒-浮游微藻吸附法,用携带WSSV的微藻投喂美丽猛水蚤(Nitocrasp.)5 d,经套式PCR检测,美丽猛水蚤为病毒阳性。这说明浮游微藻可以携带并且传播WSSV。

Phytoplankton is the base of the food web in pond cultures. In addition, they remove small particles in culture water, ineluding viruses which are excreted by the infected animals, maintain the stable environment condition for culture. In present study, the possibility was studied that four micrealgea（ Isochrysis zhanjiangensis , Platymonas subcordiformis, D unaliella salina and Skeletonema costatum） may act as a carrier or vector of white spot syndrome virus. After mixed with the WSSV in column, it was found that the micre-algae could carry WSSV within 96 h. The harpacticoid copepod Nitocra sp. could infect WSSV by filtering the phytoplanktons which were carrying WSSV. The results showed that the phytoplankton could be a carrier and vector of white spot syndrome virus.