摘要
介绍了应用模拟移动床技术分离高含量人参皂甙Rb1的工艺。确定了模拟移动床分离人参皂甙Rb1的操作条件,洗脱剂:V(甲醇)∶V(水)=45∶55;模式:1-4-3;切换时间:360 s;洗脱流速:60 mL/min;萃取液流速:30mL/min;进样流速:3 mL/min;样品质量浓度:0.2 g/mL。讨论了影响模拟移动床分离的主要因素,并与单柱分离工艺进行了比较。
For separation of ginsenoside Rb1, the working parameters of simulated moving bed chromatography ( SMBC ) are set as follows. Mobile phase : V(methenol) : V(water) = 45:55 ; mode : 1 - 4 - 3 ; switch time :360 s ; flow rate of desorbent: 60 mL/min ; flow rate of extract :30 mL/min ; flow rate of injection rate: 3 mL/min; injection concentration: 0. 2 g/mL. The main factors which affect the separation process in SMBC were discussed and the comparision is made with the results obtained from single column process.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2007年第6期554-558,共5页
Fine Chemicals
基金
科技型中小企业技术创新基金项目(立项代码:03C26212100852)~~
关键词
人参皂甙RB1
模拟移动床色谱
萃取
中药现代化
ginsenoside Rb1
simulated moving bed chromatography
extraction
modernization of traditional Chinese medicines