人参皂甙Rb_1的模拟移动床分离

Separation of Ginsenoside Rb_1 by Simulated Moving Bed Chromatography

介绍了应用模拟移动床技术分离高含量人参皂甙Rb1的工艺。确定了模拟移动床分离人参皂甙Rb1的操作条件,洗脱剂:V(甲醇)∶V(水)=45∶55;模式:1-4-3;切换时间:360 s;洗脱流速:60 mL/min;萃取液流速:30mL/min;进样流速:3 mL/min;样品质量浓度:0.2 g/mL。讨论了影响模拟移动床分离的主要因素,并与单柱分离工艺进行了比较。

For separation of ginsenoside Rb1, the working parameters of simulated moving bed chromatography （ SMBC ） are set as follows. Mobile phase ： V（methenol） ： V（water） = 45：55 ; mode ： 1 - 4 - 3 ; switch time ：360 s ; flow rate of desorbent： 60 mL/min ; flow rate of extract ：30 mL/min ; flow rate of injection rate： 3 mL/min; injection concentration： 0. 2 g/mL. The main factors which affect the separation process in SMBC were discussed and the comparision is made with the results obtained from single column process.