改构型酸性成纤维细胞生长因子对顺铂肾损害保护作用的研究

Protective Effects of MaFGF on Renal Tubular Epithelial Cells Damaged by Cisplatin

目的:研究改构型酸性成纤维细胞生长因子(MaFGF)对顺铂(DDP)引起的体外培养的肾小管上皮细胞损害的保护作用。方法:将原代培养的肾小管上皮细胞接种于96孔培养板:(1)培养72h后加入一系列浓度的DDP,实验组在DDP作用12h后加入不同浓度MaFGF,再培养36h后用WST-8法检测细胞存活率。(2)以DDP建立损伤模型,并在加药后12h加入一定量的MaFGF,观察MaFGF对肾小管上皮细胞的保护作用。结果:(1)MaFGF能使DDP对肾小管上皮细胞的半数抑制浓度(IC50)升高。(2)DDP组与对照组比较,各生化和酶学指标差异均有统计学意义;而MaFGF+DDP组与对照组比较,SOD、GSH-Px酶活性差异无统计学意义,MDA、NO升高差异仍有统计学意义。结论:MaFGF对DDP损伤的肾小管上皮细胞有明显的保护作用。

Objective：To investigate the protective effects of MaFGF on the injury of cultured renal tubular epithelial cells induced by DDP. Methods ： These primary cells were inoculated into 96 well plates. （ 1 ） After 72h culture, DDP with different concentration was added, and MaFGF with different concentration was added further as the experimental group after 12h of the DDP addition. WST- 8 assays were performed to test the cell viability after 36h treated by MaFGF. （2）The injury model was induced by DDP. And the protective effect of MaFGF on the injured renal tubular epithelial cells was observed. Results ： （ 1 ） The concentration of DDP that inhibited 50% cell growth （IC50） was increased by MaFGF; （2） The biochemical and zymological results between the DDP group and the control group were different significantly. However, with the addition of MaFGF, the differences of SOD, GSH -Px enzyme activity between the two groups were insignificant, except for MDA and NO. Conclusion MaFGF could exert obvious protective effects on the renal tubular epithelial cells damaged by DDP.